|
KMID : 0525720170220010023
|
|
Journal of Chitin and Chitosan
2017 Volume.22 No. 1 p.23 ~ p.27
|
|
|
Effects of Deep-Sea Water on Superoxide Anion Radical Scavenging Activity of Chitosan Oligosaccharide
|
|
Lee Min-Gu
Chun So-Young
Lee Kyu-Shik
Nam Kyung-Soo
|
|
|
Abstract
|
|
|
|
In this study, we investigated the effect of deep-sea water (DSW) on chitosan oligosaccharide (COS-L)-mediated superoxide anion radical scavenging activity using electron spin resonance (ESR) spectroscopy. At first, we observed anti-oxidative activity of COS-L in a concentration-dependent manner (25~750 μg/mL). Also, superoxide scavenging activity of DSW was found in a hardness-dependent manner (hardness 250~1,000). The concentrations of COS-L and the hardness of DSW used in the experiment were not found to be toxic to the HaCat cells, respectively. Based on the results, we evaluated effects of DSW on superoxide scavenging activity of COS-L (250 μg/mL). Then, COS-L was dissolved in DSW of various hardness and then the mixtures were added in 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) solution. Next, the mixtures added in DMPO solution were exposed with ultraviolet light to produce superoxide. The result showed that superoxide anion radical scavenging activity induced by COS-L (250 μg/mL) were significantly increased by DSW with hardness dependently (the range of hardness was 250, 500, 750 and 1,000, respectively). These data suggest that mixtures of COS-L (250 μg/mL) and DSW should increase superoxide scavenging activity by spin trap. Consequently, DSW effectively enhanced superoxide scavenging activity of COS-L.
|
|
|
KEYWORD
|
|
|
Deep-sea water, Chitosan oligosaccharide, Antioxidant activity, Superoxide scavenging activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|